Jornal Vascular Brasileiro
https://www.jvascbras.org/article/doi/10.1590/1677-5449.210080
Jornal Vascular Brasileiro
Original Article

Abdominal aortic aneurysm model in swine with bovine pericardium patch

Modelo de aneurisma de aorta abdominal em suínos com saco pericárdico bovino

Sílvio César Perini; Leonardo Henrique Bertolucci; Ana Paula Donadello Martins; Luís Henrique Gil França; Celso Curcio Aveline; Adamastor Humberto Pereira

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Abstract

Abstract: Background: Aneurysm repair using endovascular techniques has grown in importance as materials have improved. Studies of endovascular prostheses require experimental models that reproduce anatomic and pathophysiological characteristics of human aneurysms.

Objectives: To describe a porcine model of abdominal aortic aneurysm.

Methods: This prospective cohort study used eleven Large White female pigs with a mean age of 12 weeks in two study phases. In phase I, the aneurysm was produced with a bovine pericardium patch by retroperitoneal surgery conducted under general anesthesia. In phase II, 15 days later, the animals underwent arteriography and were then euthanized before specimens were removed for histological analysis.

Results: Formation of parietal thrombus was observed in all animals. Microscopic analysis showed calcifications around thrombus in 82% of the animals. There was lymphoplasmacytic infiltration in the graft and adjacent area, with fibrosis in nine animals. Three pigs had substantial myointimal thickening, and eight had microcalcifications. Mortality was zero, and there were no ruptures, ischemia, or surgery site infections.

Conclusions: This is a unique model, using inexpensive, biocompatible material. Bovine pericardium is easy for the surgeon to handle and has very similar characteristics to autologous tissue in terms of integration with the cell wall.

Keywords

abdominal aortic aneurysm, aortic aneurysm, aneurysm

Resumo

Resumo: Contexto: A correção de um aneurisma por meio de técnicas endovasculares tem ganhado importância com o aprimoramento dos materiais. O estudo das próteses endovasculares requer modelos experimentais que reproduzam as características anatômicas e fisiopatológicas dos aneurismas humanos.

Objetivos: Descrever um modelo suíno de aneurisma de aorta abdominal.

Métodos: Este estudo de coorte prospectivo utilizou 11 porcas da raça Large White com idade média de 12 semanas nas duas fases do estudo. Na fase I, o aneurisma foi produzido com um saco de pericárdio bovino através de cirurgia por via retroperitoneal sob anestesia geral; na fase II, 15 dias após a fase I, os animais foram submetidos a arteriografia e, posteriormente, a eutanásia, sendo os espécimes retirados para análise histológica.

Resultados: A formação de trombo parietal foi observada em todos os animais. A análise microscópica mostrou calcificações ao redor do trombo em 82% dos animais. Havia infiltrado linfoplasmocitário no enxerto e na área adjacente, com fibrose em nove animais. Três porcos tinham espessamento miointimal substancial, e oito tinham microcalcificações. A mortalidade foi zero, e não houve rupturas, isquemia ou infecção no local da cirurgia.

Conclusões: É um modelo único, com material biocompatível e de baixo custo. O pericárdio bovino é de fácil manuseio pelo cirurgião e possui características muito semelhantes ao tecido autólogo em sua integração com a parede celular.
 

Palavras-chave

aneurisma de aorta abdominal, aneurisma de aorta, aneurisma

References

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Submitted date:
03/29/2021

Accepted date:
06/10/2021

613f47dea9539518d2679634 jvb Articles
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